TITLE
© 2013
Human Gametes and Preimplantation Embryos
Assessment and Diagnosis
Autors
>> Editors: David K. Gardner, Denny Sakkas, Emre Seli, Dagan Wells
ISBN
ISBN: 978-1-4614-6650-5 (Print) 978-1-4614-6651-2 (Online)
About
In recent years, the advancing science and increasing availability of assisted reproduction have given new hope to infertile couples. However, the use of IVF and ART has also led to marked increases in the number of multiple-infant live births. This poses a public health concern, as these neonates have a higher rate of pre-term delivery, compromising their survival chances and increasing their risk of lifelong disability. By optimizing the selection of gametes and embryos with high probabilities of implantation, it is possible to reduce the number of embryos transferred and, by extension, the number of high-risk multiple gestations, while maintaining or increasing pregnancy rates.
Human Gametes and Preimplantation Embryos: Assessment and Diagnosis provides a broad yet concise overview of established and developing methodologies for assessment of gamete and embryo viability in assisted reproduction. This book elucidates the best practices for precisely selecting viable specimens based on morphology and cleavage rate and covers the spectrum of emerging adjunctive technologies for predicting reproductive potential. The authors present their extensive knowledge of “omics” approaches (genomics, transcriptomics, proteomics, and metabolomics), with unbiased delineation of the associated advantages and potential pitfalls. This valuable clinical resource is well suited to infertility specialists, Ob/Gyn physicians, IVF laboratory technicians, and researchers in the fields of embryology and reproductive medicine
Table of Contents
(26 chapters)
1. Current Practices and Regulations for Embryo Transfer Worldwide: Implications on IVF Outcome
2. Analysis of the Zona Pellucida as an Indicator of Oocyte Developmental Potential
3. Morphological Assessment of Oocytes, Pronuclear and Cleavage Stage Embryos
4. Morphological Assessment of Blastocyst Stage Embryos: Types of Grading Systems and Their Reported Outcomes
5. Real-Time Imaging Strategies to Improve Morphological Assessment
6. Limitations and Benefits of Morphologic Embryo Assessment Strategies: How Far Can Morphological Assessment Go in the Identification of Viable Embryos?
7. Sperm Morphologic Characteristics and Their Impact on Embryo Quality and Pregnancy Outcome
8. Objective Biomarkers of Sperm Development and Fertility: Assessment of Sperm-Zona Pellucida Binding Ability and Hyaluronic Acid-Mediated Selection of Sperm for ICSI Fertilization
9. Selecting the Best Sperm and Its Implications in Clinical Practice
10. The Origins of Aneuploidy in Human Embryos
11. Preimplantation Genetic Screening and Diagnosis Using Fluorescent In Situ Hybridization (FISH)
12. Single Nucleotide Polymorphisms and Next Generation Sequencing
13. Use of Comparative Genomic Hybridisation (CGH) and Microarray-CGH for Preimplantation Genetic Screening
14. Quantitative SNP Array and Real-Time PCR-Based Human Preimplantation Embryo Aneuploidy Screening
15. Regulation of Gene Expression in the Oocyte and Early Embryo: Implications for Transcriptomic and Proteomic Embryo Assessment
16. Transcriptomics Technology: Promise and Potential Pitfalls
17. Transcriptomic Analysis of Cumulus and Granulosa Cells as a Marker of Embryo Viability
18. Mass Spectrometry, Proteomics, and the Study of Sperm Cell Biology
19. Proteomic Analysis of Embryo Viability
20. Metabolism of the Viable Human Embryo
21. Analysis of Respiration as an Indicator of Oocyte and Embryo Developmental Potential
22. Mitochondrial Activity as a Biomarker of Gamete and Embryo Health
23. Carbohydrate Analysis and Embryo Viability
24. Identification of Viable Embryos by Noninvasive Measurement of Amino Acids in Culture Media
25. Metabolomic Profiling of Embryos Using Spectroscopy
26. Microfluidic Devices for the Analysis of Gamete and Embryo Physiology
